Raman Activated Single Cell Droplet Sorting Technology
A single cell is the basic unit of the function and evolution of cell life on earth. Single-cell precision high-throughput functional sorting is an important tool to analyze the heterogeneity mechanism of life systems and explore the dark matter of natural microorganisms. Single cell Raman spectroscopy (SCRS) can reveal the inherent chemical composition of cells without labeling and damage. Therefore, Raman activated cell sorting technology (RACS) has attracted increasing attention.
The researchers proposed that after single cells are wrapped in droplets, ultra-high-throughput sorting can be achieved through coupling dielectrics. Droplet packaging can not only protect cells from damage during the sorting process, but also seamlessly connect with the cell culture after sorting, the extraction and analysis of DNA, RNA, protein, etc. Therefore, RADS technology has broad application prospects.
The Ramanome technology based on single-cell Raman imaging can identify and analyze almost unlimited cell functions non-labeled and non-destructively. RADS coupled with Raman group technology will be capable of high-throughput sorting of a wide range of cell functions, thereby allowing downstream culture or omics analysis of single cells with specific functions.
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